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04-20-2024, 08:00 PM
(This post was last modified: 04-20-2024, 08:14 PM by rafc.)
Ancient DNA and paleoproteomic analysis on Roman Imperial-era individuals from Histria, Romania
Sterling L. Wright et al.
https://www.sciencedirect.com/science/ar...9X2400138X
https://www.researchgate.net/publication...ia_Romania
ilabv was so kind to procure the G25 coordinates:
Quote:histria:SRR26291258,0.117238,0.11577,0.028661,0.016473,-0.001231,0.017012,0.004935,-0.009461,-0.019634,-0.020228,-0.01088,0.003297,-0.019029,-0.012799,0.00095,-0.001326,0.009127,-0.00038,0.000628,0.003752,0.007112,0.014838,0.015159,0.001807,-0.011496
histria:SRR26291260,0.114961,0.153345,-0.013576,-0.061047,0.004616,-0.021196,0.009635,-0.006923,0.009204,0.025878,-0.005684,0.005695,-0.004906,-0.011973,-0.002714,0.007558,0.018254,0.00266,0.00817,0.002501,-0.004866,-0.002597,0.002711,-0.001084,-0.000479
histria:SRR26291262,0.111547,0.157407,-0.033941,-0.076874,0.011694,-0.042391,0,-0.005769,0.008999,0.031345,0.016239,0.013788,-0.009068,0.000826,-0.021715,-0.019093,-0.004433,0.001394,-0.00264,-0.014007,-0.013975,-0.008408,-0.004067,-0.001807,0.008622
histria:SRR26291264,0.122929,0.140143,0.017348,-0.017442,0.032314,-0.006136,-0.010575,-0.004384,0.004704,0.023691,0.005684,0.002248,-0.009812,-0.00812,-0.000679,0.007027,0.007302,0.005194,0.004148,-0.006878,-0.010107,0.006059,-0.006162,0.004217,-0.001676
Two of the samples seem very East-Med, not very surprising off course. One sample plots exactly as the EIA MJ-12 (UKR_Cimmerian_o) and LIA Scy197 (Scythian_MDA) & co. So seems there was a long term continuity in that area. the last one actually plots like some of the intermediate Moldavian Scyth samples:
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04-20-2024, 08:05 PM
(This post was last modified: 04-20-2024, 08:07 PM by AimSmall.)
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There is an incredible amount of variation in the proportions assigned to the "Steppe_Proto_Yamnaya" component or the "Yamnaya_RUS_Samara" component.
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04-20-2024, 08:15 PM
(This post was last modified: 04-20-2024, 08:16 PM by AimSmall.)
This one adds rafc's two identified close samples.
Note: MJ12 has 16% coverage and SCY197 has 20.85%
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(04-20-2024, 08:09 PM)Ebizur Wrote: There is an incredible amount of variation in the proportions assigned to the "Steppe_Proto_Yamnaya" component or the "Yamnaya_RUS_Samara" component.
The first run is against 10K years ago. Yamnaya are about 5K or 6K years younger. Just how Tomenable did his calculator.
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(04-20-2024, 08:15 PM)AimSmall Wrote: This one adds rafc's two identified close samples.
Note: MJ12 has 16% coverage and SCY197 has 20.85%
Thanks, this nicely illustrates how close SRR26291264 is to those two samples who lived nearby, but one probably about 500-700 years earlier and the other 1100-1300 earlier. He clearly is very likely a local.
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Different look using Tomenable's 6th Century BCE dataset.
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04-20-2024, 09:08 PM
(This post was last modified: 04-20-2024, 09:09 PM by AimSmall.)
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That reminds on the comment from the Bulgarian forum, which predicts Southern Bulgaria becoming Anatolian shifted since the Hellenistic period. Clearly, the coastal region of the Western Black Sea would trend towards the same, since it was as Greek colony:
Quote:Histria or Istros (Ancient Greek: Ἰστρίη, Thracian river god, Danube), was founded as a Greek colony or polis (πόλις, city) near the mouth of the Danube (known as Ister in Ancient Greek) on the western coast of the Black Sea. It is near the modern town of Istria. It was the first urban settlement on today's Romanian territory when founded by Milesian settlers in the 7th century BC. It was under Roman rule from the 1st to 3rd centuries AD.
https://en.wikipedia.org/wiki/Histria_(ancient_city)
Some of those present could be Dacians or Scytho-Sarmatians though, partially at least. No known haplogroups yet?
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¿ what its greek anatolian or roman imperal ?
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ROM_11D is the only paper sample with a radiocarbon-date
dating to 433-566 CE ie the late late-antiquity though still abit pre Justinian; while ROM_9C gets an arch. date per grave goods to the 3rd-4th c. which could still fall into the early late-antiquity ~Diocletian-Constantine
overall a familiar sight in yet another prov and i think we are now getting a pretty good idea of how important Hellenistic Anatolia was for the Rom/Byz period and its profile, eg all we see in Italy is a massive influx of Anatolians and a dissolved IA cluster shifted to Anatolians (ie the real overlap) for all samples 0-200 CE (incl Pompeii f1R) and in Naissus the sample R6769 dates to 26-126 calCE, not to mention the entire data from Viminatium (cf paper); keeping in mind that the Danube was a major expansion and the most important frontier
despite Histria leaning to the late-antiquity the sample SRR26291260 is still very reminiscent of the 'shifted to' cluster in Roman Italy, judging by the ph2ter maps (# 266) and closest ancient inds on Vahaduo; not knowing which sample ID fits which G25 ID its still pos that the sample is from an earlier time since the arch. timeframe of the overall site seems to be the 2nd-6th c.
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04-20-2024, 11:43 PM
(This post was last modified: 04-20-2024, 11:49 PM by alexfritz.)
(04-20-2024, 10:37 PM)HDG33 Wrote: ¿ what its greek anatolian or roman imperal ?
Greek-Anatolian aka Hellenistic-Anatolian would be samples labeled TUR_Anc from the 'Southern-Arc' paper (Lazaridis 2022) and Roman Imperial (cf Viminatium paper) got a definition from the Reich-lab as a cluster of all C5 samples (xR45), all C6 samples (xR111) and the C4 samples R76 R75 R73 R41 R38 R126 from the Antonio/Moots paper
atleast for the latter alot more samples post-Antonio/Moots can be added from Italy and the Balkans eg
Show Content
Spoilerpre-print p5 Olalde 2021 The other major cluster (44% of the samples from Viminacium between 1-250 CE) isrepresented by individuals who projected towards ancient and present-day Eastern Mediterranean groups in PCA (Figure 1A), close to ancient individuals from Rome during Imperial times 3.
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04-21-2024, 01:17 AM
(This post was last modified: 04-21-2024, 01:31 AM by AimSmall.)
Trying to correlate the RAW data sample names which G25 inherited....
The code used and some results are published at GitHub
https://github.com/SterlingLWright/Histr...tadata.csv
https://github.com/SterlingLWright/Histr...esults.csv
I was able to make this crosswalk
SRR26291264 : ROM_10D
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04-21-2024, 06:24 AM
(This post was last modified: 04-21-2024, 08:44 AM by rafc.)
(04-21-2024, 01:17 AM)AimSmall Wrote: Trying to correlate the RAW data sample names which G25 inherited....
The code used and some results are published at GitHub
https://github.com/SterlingLWright/Histr...tadata.csv
https://github.com/SterlingLWright/Histr...esults.csv
I was able to make this crosswalk
SRR26291264 : ROM_10D
Thanks. I guess to identify the others we need the published paper, the pre-print only mentioned four out of the 10 samples they finally used. If someone can access the published paper and put the table with samples here, that would be welcome.
Edit: here is a presentation:
https://www.youtube.com/watch?v=3hpWYCg6TAg
He says they only succeeded with 4 samples. I assume they re-ran the other samples afterwards. The csv-files only list 8. So maybe the files are for a completely new paper ...
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04-21-2024, 12:21 PM
(This post was last modified: 04-21-2024, 12:27 PM by corrigendum.)
(04-20-2024, 08:00 PM)rafc Wrote: Two of the samples seem very East-Med, not very surprising off course. One sample plots exactly as the EIA MJ-12 (UKR_Cimmerian_o) and LIA Scy197 (Scythian_MDA) & co. So seems there was a long term continuity in that area. the last one actually plots like some of the intermediate Moldavian Scyth samples:
SCY197 might be from a later era because he couldn't be dated.
In any case, SRR26291264 is closer to west Balkan samples than to Scy197 or MJ-12, hence I don't think that it represents a local profile (although I don't consider scy197 to represent a local profile as well, but a west-central Balkan one):
Code: Distance to: SRR26291264
0.02656263 Austria_Klosterneuburg_Roman_oLevant.SG:R10654.SG
0.02673534 Italy_Medieval_EarlyModern_oCentralEuropean.SG:R55.SG
0.02683323 Hungary_Langobard_o1.SG:SZ36.SG
[b]0.02787681 Montenegro_MLBA:I13775[/b]
[b]0.02855638 Croatia_BA:I18748[/b]
0.02871322 Hungary_MidAvar:SSD-144.SG
[b]0.02878922 Croatia_MBA_Cetina:I11843[/b]
[b]0.02919788 Montenegro_MLBA:I13167[/b]
[b]0.02922306 Croatia_Medieval:I15743[/b]
0.02956592 Hungary_Langobard_o1.SG:SZ43.SG
[b]0.03007455 Croatia_MBA:I5073[/b]
[b]0.03051983 Slovakia_IA_Vekerzug:I11721[/b]
[b]0.03100371 Croatia_MBA:I4331[/b]
0.03108378 Hungary_LateAvar:SZKT-265.SG
0.03153132 Macedonia_IA:I8112
0.03168200 Hungary_LateAvar:JHT-30.SG
0.03227690 Montenegro_MLBA:I13169
0.03234870 Albania_BA_IA:I14690
0.03237701 Italy_Tuscany_Grosseto_EarlyMedieval:POP001
0.03251785 Hungary_LateAvar:HH-10.SG
0.03257396 Albania_BA_IA:I16251
0.03257759 Hungary_MidAvar:SZM-38.SG
0.03270051 Macedonia_IA:I10384
0.03329560 Montenegro_MLBA:I13168
0.03335230 Macedonia_IA:I10388
0.03351113 Croatia_Zadar_Roman.SG:R3747.SG
0.03398156 Montenegro_MLBA:I13171
0.03402947 Croatia_Popova_CA.SG:POP39_noUDG.SG
0.03406798 Slovakia_IA_Vekerzug:I12099
0.03408744 Serbia_Viminacium_Roman_elite_3.SG:R9669.SG
0.03409905 Croatia_LBA_EIA:I26742
0.03414620 Macedonia_BA:I7231
0.03418433 Hungary_Langobard_o1:SZ32
0.03446555 Hungary_Conqueror_Elite:SE-114.SG
0.03446814 Croatia_SisakPogorelec_Roman.SG:R2041.SG
[b]0.04257057 MJ-12_noUDG.SG[/b]
[b]0.04436875 scy197_noUDG.SG[/b]
SRR26291260 represents local Thracian and Greco-Anatolian ancestry.
SRR26291262 is an individual of East Med origin.
SRR26291258 originates from one of the steppe-derived groups which got there from Ukraine.
Overall, a very diverse site - as would be expected in any urban center of the Roman era. 4/4 samples represent different trajectories of ancestry.
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