01-18-2024, 12:01 AM
Analyses of complete genomes of 10 French Late Mesolithic individuals form the sites of Hoedic, Téviec and Champigny
Abstract
Since the early Holocene, western and central Europe was inhabited by a genetically distinct group of Western Hunter-Gatherers (WHGs). These groups were eventually replaced and assimilated by the incoming Neolithic farmers. The western Atlantic façade was home to some of the last Mesolithic sites of mainland Europe, represented by the iconic open-air sites at Hoedic and Téviec in southern Brittany, France. These sites are known for the unusually well-preserved and rich burials. Genomic studies of Mesolithic European hunter-gatherers have been limited to single or a few individuals per site and our understanding of the social dynamics of the last Mesolithic hunter-gatherers of Europe and their interactions with incoming farmers is limited. We sequenced and analyzed the complete genomes of 10 individuals from the Late Mesolithic sites of Hoedic, Téviec, and Champigny, in France, four of which sequenced to between 23- and 8-times genome coverage. The analysis of genomic, chronological and dietary data revealed that the Late Mesolithic populations in Brittany maintained distinct social units within a network of exchanging mates. This resulted in low intra-group biological relatedness that prevented consanguineous mating, despite the small population size of the Late Mesolithic groups. We found no genetic ancestry from Neolithic farmers in the analyzed hunter-gatherers, even though some of them may have coexisted with the first farming groups in neighboring regions. Hence, contrary to previous conclusions based on stable isotope data from the same sites, the Late Mesolithic forager community was limited in mate-exchange to neighboring hunter-gatherer groups, to the exclusion of Neolithic farmers.
https://www.ebi.ac.uk/ena/browser/view/PRJEB71770
G25 (no labels)
Y-DNA with Yleaf (two read minimum)
Abstract
Since the early Holocene, western and central Europe was inhabited by a genetically distinct group of Western Hunter-Gatherers (WHGs). These groups were eventually replaced and assimilated by the incoming Neolithic farmers. The western Atlantic façade was home to some of the last Mesolithic sites of mainland Europe, represented by the iconic open-air sites at Hoedic and Téviec in southern Brittany, France. These sites are known for the unusually well-preserved and rich burials. Genomic studies of Mesolithic European hunter-gatherers have been limited to single or a few individuals per site and our understanding of the social dynamics of the last Mesolithic hunter-gatherers of Europe and their interactions with incoming farmers is limited. We sequenced and analyzed the complete genomes of 10 individuals from the Late Mesolithic sites of Hoedic, Téviec, and Champigny, in France, four of which sequenced to between 23- and 8-times genome coverage. The analysis of genomic, chronological and dietary data revealed that the Late Mesolithic populations in Brittany maintained distinct social units within a network of exchanging mates. This resulted in low intra-group biological relatedness that prevented consanguineous mating, despite the small population size of the Late Mesolithic groups. We found no genetic ancestry from Neolithic farmers in the analyzed hunter-gatherers, even though some of them may have coexisted with the first farming groups in neighboring regions. Hence, contrary to previous conclusions based on stable isotope data from the same sites, the Late Mesolithic forager community was limited in mate-exchange to neighboring hunter-gatherer groups, to the exclusion of Neolithic farmers.
https://www.ebi.ac.uk/ena/browser/view/PRJEB71770
G25 (no labels)
Code:
Scaled
various:hoe001,0.112685,0.103584,0.194971,0.200261,0.145566,0.061914,0.011751,0.039229,0.098171,0.025695,-0.015914,-0.009891,0.022745,-0.002752,0.065417,0.066825,0.0103,0.009882,-0.011313,0.054276,0.11879,0.019537,-0.05731,-0.181351,0.024788
various:hoe002,0.121791,0.111708,0.191577,0.197677,0.163415,0.051874,0.017861,0.038075,0.098785,0.016037,-0.014777,-0.010641,0.014569,-0.012524,0.065146,0.065764,0.004563,0.008361,-0.01169,0.057027,0.109557,0.016569,-0.052997,-0.177013,0.026345
various:hoe003,0.125205,0.105615,0.199497,0.198646,0.165877,0.054941,0.021621,0.039921,0.097149,0.014397,-0.0177,-0.014237,0.014717,-0.000826,0.060396,0.067621,0.005346,0.004307,-0.006536,0.061029,0.121036,0.010881,-0.058912,-0.187014,0.026465
various:hoe004,0.124067,0.119832,0.193463,0.199292,0.159414,0.058567,0.023736,0.044075,0.094081,0.020775,-0.0177,-0.015286,0.01665,0.005092,0.066232,0.047997,-0.029076,0.010008,-0.006034,0.054026,0.106687,0.020774,-0.051641,-0.174964,0.021076
various:hoe005,0.119514,0.114755,0.198365,0.192832,0.170493,0.059683,0.017156,0.047536,0.099603,0.014397,-0.014128,-0.014387,0.014866,0.001651,0.070032,0.043755,-0.026989,0.014949,-0.006662,0.054526,0.122783,0.016075,-0.051148,-0.182074,0.023351
various:stp001,0.124067,0.107646,0.193463,0.203814,0.159107,0.066934,0.018096,0.040614,0.09858,0.008018,-0.018837,-0.014837,0.015609,-0.003716,0.066503,0.048263,-0.023469,0.012289,-0.004651,0.053776,0.11068,0.009274,-0.059529,-0.178218,0.024908
various:tev001,0.117238,0.110693,0.19384,0.190894,0.160645,0.053547,0.012691,0.043844,0.103489,0.021686,-0.020461,-0.018583,0.011001,-0.010459,0.06311,0.061919,0.002738,0.007095,-0.00905,0.06178,0.114922,0.011623,-0.054475,-0.189906,0.021196
various:tev002,0.127482,0.105615,0.18592,0.201553,0.166185,0.064145,0.00752,0.027922,0.096944,0.02041,-0.019324,-0.016036,0.011596,-0.012661,0.063924,0.063113,0.002868,0.010135,-0.008422,0.064906,0.117917,0.006677,-0.057064,-0.178941,0.02371
various:tev003,0.121791,0.108662,0.1912,0.191863,0.166493,0.058009,0.015981,0.03023,0.095308,0.015308,-0.019811,-0.014687,0.018434,-0.005918,0.06786,0.067223,-0.004433,0.013682,-0.009302,0.054276,0.115671,0.011252,-0.055092,-0.183038,0.022513
Raw
various:hoe001,0.0099,0.0102,0.0517,0.062,0.0473,0.0222,0.005,0.017,0.048,0.0141,-0.0098,-0.0066,0.0153,-0.002,0.0482,0.0504,0.0079,0.0078,-0.009,0.0434,0.0952,0.0158,-0.0465,-0.1505,0.0207
various:hoe002,0.0107,0.011,0.0508,0.0612,0.0531,0.0186,0.0076,0.0165,0.0483,0.0088,-0.0091,-0.0071,0.0098,-0.0091,0.048,0.0496,0.0035,0.0066,-0.0093,0.0456,0.0878,0.0134,-0.043,-0.1469,0.022
various:hoe003,0.011,0.0104,0.0529,0.0615,0.0539,0.0197,0.0092,0.0173,0.0475,0.0079,-0.0109,-0.0095,0.0099,-0.0006,0.0445,0.051,0.0041,0.0034,-0.0052,0.0488,0.097,0.0088,-0.0478,-0.1552,0.0221
various:hoe004,0.0109,0.0118,0.0513,0.0617,0.0518,0.021,0.0101,0.0191,0.046,0.0114,-0.0109,-0.0102,0.0112,0.0037,0.0488,0.0362,-0.0223,0.0079,-0.0048,0.0432,0.0855,0.0168,-0.0419,-0.1452,0.0176
various:hoe005,0.0105,0.0113,0.0526,0.0597,0.0554,0.0214,0.0073,0.0206,0.0487,0.0079,-0.0087,-0.0096,0.01,0.0012,0.0516,0.033,-0.0207,0.0118,-0.0053,0.0436,0.0984,0.013,-0.0415,-0.1511,0.0195
various:stp001,0.0109,0.0106,0.0513,0.0631,0.0517,0.024,0.0077,0.0176,0.0482,0.0044,-0.0116,-0.0099,0.0105,-0.0027,0.049,0.0364,-0.018,0.0097,-0.0037,0.043,0.0887,0.0075,-0.0483,-0.1479,0.0208
various:tev001,0.0103,0.0109,0.0514,0.0591,0.0522,0.0192,0.0054,0.019,0.0506,0.0119,-0.0126,-0.0124,0.0074,-0.0076,0.0465,0.0467,0.0021,0.0056,-0.0072,0.0494,0.0921,0.0094,-0.0442,-0.1576,0.0177
various:tev002,0.0112,0.0104,0.0493,0.0624,0.054,0.023,0.0032,0.0121,0.0474,0.0112,-0.0119,-0.0107,0.0078,-0.0092,0.0471,0.0476,0.0022,0.008,-0.0067,0.0519,0.0945,0.0054,-0.0463,-0.1485,0.0198
various:tev003,0.0107,0.0107,0.0507,0.0594,0.0541,0.0208,0.0068,0.0131,0.0466,0.0084,-0.0122,-0.0098,0.0124,-0.0043,0.05,0.0507,-0.0034,0.0108,-0.0074,0.0434,0.0927,0.0091,-0.0447,-0.1519,0.0188
Y-DNA with Yleaf (two read minimum)
Code:
Sample_name Hg Hg_marker Total_reads Valid_markers
hoe001 NA 13227633 479
hoe002 I-Y3104*(xL621,L161) S7721;Z2589;etc. 325651200 94608
hoe003 I-Y3104*(xL621,L161) YP202;Y3109;etc. 2137673885 128055
hoe004 R-L52 CTS7650 402728620 14992
hoe005 R-L52 CTS7650 652343679 18903
hoe006 NA 2267412 11
stp001 I-CTS616*(xCTS10057) CTS9139;CTS11271;etc. 1021738494 127816
tev001 I-L460*(xM436) AM00522;Y8776 250203247 61589
tev002 A1*(xA1a) Y9619;YP3891 21552051 1717
tev003 I-Y3104*(xL621,L161) Z2585;YP212;etc. 2066566932 128063